Taconic Biosciences offers Transgene Mapping Analysis in partnership with Cergentis. This service uses targeted locus amplification technology, developed by Cergentis, to identify the exact integration site of a transgene that is randomly integrated into the genome of a mouse or rat via pronuclear injection.
Taconic provides end-to-end solution from sample preparation to final reporting which includes:
Precise identification of all transgene integration sites
Characterization of any structural changes in the transgene and genomic regions surrounding the transgene
Structural changes in the transgene sequence
Any Single Nucleotide Variants within the transgene
Founder line recommendations and breeding plan optimization as applicable
Recommended genotyping protocols that bridge the integration site and allow for genotyping specific integration sites and accurately differentiate homozygous vs heterozygous animals. In some cases, developing genotyping protocols may not be possible due to the nature and the site of transgene integration.