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Flow Cytometric Analysis of Humanized Immune System Mice

Humanized immune system (HIS) mice are powerful tools for modeling the immune system, with applications in immuno-oncology, autoimmune disease and more. While there are many types of HIS models, flow cytometry is commonly used to analyze nearly all HIS models. 

Despite how widely flow cytometry is used with HIS mice, there are no standardized protocols, and this can create confusion. Dr. Smith will discuss a wide range of topics related to flow cytometry and HIS mice including: design of flow cytometry panels for extended lineage HIS mice including assessment of the myeloid compartment, important considerations for blood sampling during HIS mouse experiments, chimerism calculation methods, application of spectral flow cytometry to HIS mouse analysis, and the types of data provided with shipment of Taconic HIS mice. 

Watch this virtual presentation as Dr. Nicholas Smith, Manager, Flow Cytometry for Taconic Biosciences, provides a detailed overview of flow cytometric analysis for HIS mice, including the application of cutting-edge spectral flow cytometry.


View this webinar to learn about:

  • Important considerations for design of panels for flow cytometric analysis of HIS mice
  • What type of data users can expect with shipments of HIS mice from Taconic Biosciences
  • How the timing of development of certain cell populations in HIS mice impacts study design
  • The various uses of flow cytometry, ranging from a basic QC step for assessing successful engraftment of human cells to analysis of human immune cells infiltrating tumors in complex oncology experimental settings.

Dr. Nicholas Smith

Manager, Flow Cytometry

Dr. Nicholas Smith is the manager of Taconic’s flow cytometry lab. He obtained a BA in biology from the University of Vermont, and a PhD in immunology from the University of Colorado Anschutz Medical Campus. Nick performed postdoctoral research at Brigham and Women’s Hospital and Suny Upstate Medical University. His graduate and postdoctoral research utilized flow cytometry to model in vitro, ex vivo, and in vivo herpesvirus infection to understand viral tropism and changes in cell surface marker expression during infection.

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