Delivering Results Faster on Custom Mouse Model Engineering Projects with the ExpressMODEL® CRISPR Platform
This webinar will present the workflow for CRISPR/Cas9-based custom genetically engineered mouse models, overviewing technical and programmatic considerations. This will include a brief history of the use of the technology and the progression to today’s applications in model generation and recommendations for use. We will also review the importance of mosaicism assessment and screening for off-target mutations when using CRISPR/Cas9 and how data-informed selection of gene-edited founders is advantageous to model generation projects and study programs that require them.
We will outline details on the concept behind the ExpressMODEL ® CRISPR Platform and our approach to using next generation sequencing for molecular characterization of founder that includes mosaicism determination and analysis of predicted potential off-target sites. We will present how we use NGS data to ensure quality of the model and to shorten the time to study results. We will include examples that highlight the cost and time-saving benefits of choosing ExpressMODEL ®
In addition, we will show how we apply the ExpressMODEL ® concept to two other model generation methodologies, embryonic stem cell targeting and random integration transgenesis.
View this webinar to learn:
- The workflow for custom mouse models generated using CRISPR/Cas9
- How Taconic uses next generation sequencing to assess mosaicism in founder mice and to screen for potential off-target mutations
- How molecular characterization of founder mice and germline transmission by in vitro fertilization reduces the time to results for programs requiring mouse models made by CRISPR/Cas9
Kenn Albrecht, PhD
Associate Director, Scientific Services | Custom Model Generation Solutions
Engaged in mouse model generation and characterization for some 25 years, Kenn is broadly trained in classical genetics, molecular genetics, genomics and developmental biology. He is an adjunct assistant professor at Boston University School of Medicine and holds a B.S. in biology from the State University of New York at Stony Brook and a Ph.D. in genetics from the University of Connecticut.