An Introduction to Humanized Immune System (HIS) Mice - Part I in a three-part series
Humanized Immune System (HIS) mouse models are useful preclinical tools to further understand the immune system. HIS mice have many applications, including immuno-oncology, infectious disease, allergy and more. Immunodeficient host strains form the base of HIS models, and the severely immunodeficient CIEA NOG mouse® is commonly used for both xenograft and human cell engraftment studies. The NOG mouse lacks mature T, B, and NK cells, and it can support engraftment of different components of the human immune system. There are two basic approaches to humanize NOG mice, using either peripheral blood mononuclear cells (PBMCs), which are differentiated mature cells from adult donors, or hematopoietic stem cells (HSCs).
This webinar will cover the strengths and limitations of each approach. While these foundational HIS models have been widely exploited for drug discovery, humanization of second-generation NOG strains allows researchers to explore a greater range of questions. As immuno-oncology approaches have expanded beyond T cells, these second-generation NOGs support a range of important human cell types and evaluation of cell therapies.
View this webinar to learn about:
- How the NOG model compares to standard immunodeficient models
- How the NOG mouse and similar strains support humanization via two key approaches
- The strengths and limitations of different methods of humanization
- The importance of selection of the right model, starting from your human cell types of interest
- A brief introduction to second generation NOG strains which support engraftment and differentiation of additional human cell types
Dr. Mayara Grizotte-Lake
Field Application Scientist
Dr. Mayara Grizotte-Lake received her Bachelor of Science in Biology from UMass Dartmouth, and her Ph.D. from Brown University in Pathobiology with a focus on Molecular Microbiology and Immunology. During her Ph.D. research, she made a novel finding regarding how commensal bacteria can downregulate vitamin A metabolism. With more than ten years of in vivo experience, she has great expertise in immunology, host-microbe interaction, rodent models and vaccine research. She is currently a Field Application Scientist at Taconic.