Design, Validation and Optimization of Flow Cytometry Panels for Analysis of Humanized Immune System Mice
Flow cytometry is a powerful tool used to monitor both human and murine immune cell populations in HIS mouse research. Dive deeper into flow cytometric analysis of humanized immune system (HIS) mice in this new webinar.
Dr. Smith provides practical tips on steps involving the design of panels, including working within the instrument capabilities, assigning markers to properly assess your experimental question, and best practices in determining proper fluorophore combinations.
Watch this webinar to explore the process of optimizing and validating new flow cytometry panels, including material to use for optimization experiments, antibody titration and analysis, and the utilization of proper controls. Dr. Smith covers common difficulties and troubleshooting strategies involved with developing assays for flow cytometric analysis of HIS mice, including gating strategies and assessing rare cell populations.
Not ready for a deep dive just yet? Check out our webinar on the basics of flow cytometry for HIS mice.
In this webinar, you will:
- Develop a deeper understanding of the process of flow cytometry analysis of HIS mice, including sample prep, panel design and optimization
- Learn how to troubleshoot complex flow cytometry assays and apply proper controls
- Learn how to assess rare cell populations in HIS mice
- How to get the best quality data from your HIS mouse experiments
Nicholas Smith, PhD
Manager, Flow Cytometry
Dr. Nicholas Smith is the manager of Taconic’s flow cytometry lab. He obtained a BA in biology from the University of Vermont, and a PhD in immunology from the University of Colorado Anschutz Medical Campus. Nick performed postdoctoral research at Brigham and Women’s Hospital and Suny Upstate Medical University. His graduate and postdoctoral research utilized flow cytometry to model in vitro, ex vivo, and in vivo herpesvirus infection to understand viral tropism and changes in cell surface marker expression during infection.